On the Brink: A New Synaptic Vesicle Release Model at the Calyx of Held

How vesicle calcium sensors interact with calcium channels at synapses affects neurotransmitter release dynamics. In this issue of Neuron, Nakamura et al. (2015) propose that synaptic vesicles are tightly coupled around the perimeter of a voltage-gated calcium channel cluster

Iconography in Dialogue: Negotiating Tradition and Cultural Contact in the Art of Seventh Century Anglo-Saxon England

The seventh century in Anglo-Saxon England offers a particularly rich historical period in which to examine the material effects of cultural contact between disparate cultures. As it lacks close contemporary textual accounts of the events taking place and the cultural response to the resultant changes, the material record becomes the site upon which that dialogue plays out. This study is primarily concerned with the art of what might be considered a transitional period in Anglo-Saxon England, the seventh century, between the arrival of the papal mission from Rome in 597 CE and the beginning of the eighth century when Christianity seems to have become well established throughout much of the region. At its core, this study is intended to present an iconographic, art historical examination of the artwork produced in this historical period, focusing predominantly on decorated metalwork, specifically personal ornament, as a medium for the transmission of iconography. To that end, given the scope of the historical events and material artefacts encompassed within that time period, it will focus primarily on traditional iconography, the so-called Germanic motifs, and their persistence and resurgence in response to the incoming Mediterranean influences. The artwork, specifically the metalwork, demonstrates a valuation and retention of longstanding traditions, both iconographic and aesthetic, which seem to be in direct response to the introduction of a competing culture. This embracing of tradition does not mean that significant cultural change was occurring throughout the seventh century, nor does it necessarily indicate a societal resistance to that change; however it does reveal that there was a level of uncertainty about the changes taking place and a resultant desire for the familiar, symbolic and significant traditions of the remembered past

Glutamatergic transmission in the central nucleus of the amygdala is selectively altered in Marchigian Sardinian alcohol-preferring rats: Alcohol and CRF effects

The CRF system of the central nucleus of the amygdala (CeA) is important for the processing of anxiety, stress, and effects of acute and chronic ethanol. We previously reported that ethanol decreases evoked glutamate transmission in the CeA of Sprague Dawley rats and that ethanol dependence alters glutamate release in the CeA. Here, we examined the effects of ethanol, CRF and a CRF1 receptor antagonist on spontaneous and evoked glutamatergic transmission in CeA neurons from Wistar and Marchigian Sardinian Preferring (msP) rats, a rodent line genetically selected for excessive alcohol drinking and characterized by heightened activity of the CRF1 system. Basal spontaneous and evoked glutamate transmission in CeA neurons from msP rats was increased compared to Wistar rats. Ethanol had divergent effects, either increasing or decreasing spontaneous glutamate release in the CeA of Wistar rats. This bidirectional effect was retained in msP rats, but the magnitude of the ethanol-induced increase in glutamate release was significantly smaller. The inhibitory effect of ethanol on evoked glutamatergic transmission was similar in both strains. CRF also either increased or decreased spontaneous glutamate release in CeA neurons of Wistar rats, however, in msP rats CRF only increased glutamate release. The inhibitory effect of CRF on evoked glutamatergic transmission was also lost in neurons from msP rats. A CRF1 antagonist produced only minor effects on spontaneous glutamate transmission, which were consistent across strains, and no effects on evoked glutamate transmission. These results demonstrate that the genetically altered CRF system of msP rats results in alterations in spontaneous and stimulated glutamate signaling in the CeA that may contribute to both the anxiety and drinking behavioral phenotypes

416— The effects of early life trauma on anxiety and alcohol use is modified by environment

Early life trauma is a risk factor for later anxiety and alcohol use disorders. However, the role of the post-trauma environment on the development of such disorders is not well understood. In the present study we investigated experience-dependent changes in anxiety and alcohol use after exposure to early trauma. Young mice (day 23) were exposed to a predator odor (synthetic fox pheromone, TMT) and then reared in either standard (SE) or enriched environments (EE). Adolescent anxiety and conditioned fear were reduced in EE-males, but not EE-females. Adult mice were then tested for their preference to drink alcohol. Alcohol intake escalated across sessions for all mice, however, EE mice had overall lower levels of alcohol use. Interestingly, exposure to TMT affected alcohol preference in EE mice, but not standard mice. Findings demonstrate the environment as a developmental modifier of post-trauma anxiety and alcohol use disorders

Building Social & Emotional Competence in Early Education Classrooms through Art Therapy

This research project qualitatively explores how teachers address social-emotional learning and how educators understand and implement SEL and art therapy in early education. This research aimed to explore how art therapy theories can support teachers in their efforts to create socially and emotionally competent early education classrooms. Data collected included anonymous surveys from current kindergarten through third-grade teachers. Through data analysis, several findings suggest that art therapists, mental health practitioners, and teachers can benefit from collaboration in increasing access and training with SEL practices. These findings open new doors for further inquiry into art therapy and SEL

Overview: Solar Electric Propulsion Concept Designs for SEP Technology Demonstration Mission

JPC presentation of the Concept designs for NASA Solar Electric Propulsion Technology Demonstration mission paper. Multiple Solar Electric Propulsion Technology Demonstration Missions were developed to assess vehicle performance and estimated mission cost. Concepts ranged from a 10,000 kg spacecraft capable of delivering 4000 kg of payload to one of the Earth Moon Lagrange points in support of future human-crewed outposts to a 180 kg spacecraft capable of performing an asteroid rendezvous mission after launched to a geostationary transfer orbit as a secondary payload

Distribution of Extracellular Glutamate in the Neuropil of Hippocampus

Reported values of extracellular glutamate concentrations in the resting state depend on the method of measurement and vary ∼1000-fold. As glutamate levels in the micromolar range can cause receptor desensitization and excitotoxicity, and thus affect neuronal excitability, an accurate determination of ambient glutamate is important. Part of the variability of previous measurements may have resulted from the sampling of glutamate in different extracellular compartments, e.g., synaptic versus extrasynaptic volumes. A steep concentration gradient of glutamate between these two compartments could be maintained, for example, by high densities of glutamate transporters arrayed at the edges of synapses. We have used two photon laser scanning microscopy and electrophysiology to investigate whether extracellular glutamate is compartmentalized in acute hippocampal slices. Pharmacological blockade of NMDARs had no effect on Ca2+ transients generated in dendritic shafts or spines of CA1 pyramidal neurons by depolarization, suggesting that ambient glutamate is too low to activate a significant number of NMDARs. Furthermore, blockade of transporters did not flood the synapse with glutamate, indicating that synaptic NMDARs are not protected from high concentrations of extrasynaptic glutamate. We suggest that, in the CA1 region of hippocampus, glutamate transporters do not create a privileged space within the synapse but rather keep ambient glutamate at very low levels throughout the neuropil